[Histonet] double stainings
carl.hobbs <@t> kcl.ac.uk
Wed Sep 22 13:31:38 CDT 2004
I agree with previous two posts. DAB for chromogen of non-HIER, then
whatever you want to contrast for second Ag. Technically one should use a
different enzyme system but, if the Ags are not co-localised, I might do
HRP/DAB/Cobalt/Nickel enhanced, giving brown and blue/black. The first
reporter molecule is sufficiently masked for it not to interfere. Obviously
do parallel sections with individual detection systems just for your/their
peace of mind. Only need to do that once
Outgoing mail is certified Virus Free.
Checked by AVG anti-virus system (http://www.grisoft.com).
Version: 6.0.762 / Virus Database: 510 - Release Date: 13/09/2004
More information about the Histonet