[Histonet] Eosinophils in mouse tissue

[Bharat Mani]

Dear Todd

We do a lot of experiments on eosinophilia in mouse, ensure to perfuse the lungs before processing, am sharing our protocol -

1.	We use 10% NBF to perfuse the mouse lungs and then subsequently proceed for immersion fixation for atleast a minimum of 24-48 hours before processing the mouse lung/s tissue.
2.	Next we do a routine H&E on sections cut at 5µ.

Hope this helps

Regards

Bharat Mani
Research Scientist (Biological Research)
Glenmark Research Centre
Plot No. A/607, TTC Ind. Area,
MIDC, Mahape
Navi Mumbai - 400 709
Ph.: +92-22-5590 2491/92 Ext.: 235
Fax: +91-22-5590 2318
Email: bharatm <@t> glenmarkpharma.com <mailto:bharatm <@t> glenmarkpharma.com> 
Web: www.glenmarkpharma.com <http://www.glenmarkpharma.com> 


-----Original Message-----
From: Histology SLU [mailto:sluhisto <@t> yahoo.com] 
Sent: Friday, September 17, 2004 1:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosinophils in mouse tissue


Does anyone out there have a protocol for staining eosinophils in mouse lung?  Thanks.
 
Todd

		
---------------------------------
Do you Yahoo!?
vote.yahoo.com - Register online to vote today! _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet


DISCLAIMER:
The information contained in this email communication is intended only for the personal and confidential use of the designated recipient named above.  This message may be an attorney-client communication, and as such is privileged and confidential. If the reader of this message is not the intended recipient, you are hereby notified that you have received this communication in error, and that any review, dissemination, distribution, or copying of the message is strictly prohibited. If you have received this transmission in error, please destroy this transmission and notify Glenmark Pharmaceuticals immediately by telephone and/or send an email to supportho <@t> glenmarkpharma.com