[Histonet] Eosinophils in mouse tissue

Bharat Mani bharatm <@t> glenmarkpharma.com
Thu Sep 16 22:52:30 CDT 2004

Dear Todd

We do a lot of experiments on eosinophilia in mouse, ensure to perfuse the lungs before processing, am sharing our protocol -

1.	We use 10% NBF to perfuse the mouse lungs and then subsequently proceed for immersion fixation for atleast a minimum of 24-48 hours before processing the mouse lung/s tissue.
2.	Next we do a routine H&E on sections cut at 5µ.

Hope this helps


Bharat Mani
Research Scientist (Biological Research)
Glenmark Research Centre
Plot No. A/607, TTC Ind. Area,
MIDC, Mahape
Navi Mumbai - 400 709
Ph.: +92-22-5590 2491/92 Ext.: 235
Fax: +91-22-5590 2318
Email: bharatm <@t> glenmarkpharma.com <mailto:bharatm <@t> glenmarkpharma.com> 
Web: www.glenmarkpharma.com <http://www.glenmarkpharma.com> 

-----Original Message-----
From: Histology SLU [mailto:sluhisto <@t> yahoo.com] 
Sent: Friday, September 17, 2004 1:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosinophils in mouse tissue

Does anyone out there have a protocol for staining eosinophils in mouse lung?  Thanks.

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