[Histonet] Eosinophils in mouse tissue
bharatm <@t> glenmarkpharma.com
Thu Sep 16 22:52:30 CDT 2004
We do a lot of experiments on eosinophilia in mouse, ensure to perfuse the lungs before processing, am sharing our protocol -
1. We use 10% NBF to perfuse the mouse lungs and then subsequently proceed for immersion fixation for atleast a minimum of 24-48 hours before processing the mouse lung/s tissue.
2. Next we do a routine H&E on sections cut at 5µ.
Hope this helps
Research Scientist (Biological Research)
Glenmark Research Centre
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Email: bharatm <@t> glenmarkpharma.com <mailto:bharatm <@t> glenmarkpharma.com>
Web: www.glenmarkpharma.com <http://www.glenmarkpharma.com>
From: Histology SLU [mailto:sluhisto <@t> yahoo.com]
Sent: Friday, September 17, 2004 1:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosinophils in mouse tissue
Does anyone out there have a protocol for staining eosinophils in mouse lung? Thanks.
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