[Histonet] EDTA decal protocol?

[Garlits, John]

Hello,

I am looking for a well-established EDTA decalcification protocol to use on mouse bones for IHC.  Could anyone who does this let me know what reagents and times they use?

Thanks!

John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176