[Histonet] re" Perferred" fixative for animals
carl.hobbs <@t> kcl.ac.uk
Wed Sep 15 10:13:14 CDT 2004
In my opinion, to date, no other such fixing fluid exists......OK, I havn't
tried any of the commercial preps but if they were superior to formalin
we'd all be using it/them. I've gone thro' the range of standard fixing
fluids and also such as "Perfix" and "Sera's" fluid. None of them are
superior to Formalin regarding their preservation of antigenicity/ their
capacity for antigen retrieval. Some are equal for particular antigens eg
Perfix -fixed mouse tissue will demonstrate phosphoHistones beautifully but
not any other Ag that I'm interested in, but formalin - fixed tissue will
also demonstrate them, after AR; it will also allow for the demonstration
of many, many other Ags in the same block. This applies to mouse, rat,
dogfish, trout, fruitfly and chick, in my experience.
Why, may I ask, do you not wish to use Formalin/paraformaldehyde?
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