[Histonet] Perferred fixative for animals

Geoff McAuliffe mcauliff <@t> umdnj.edu
Tue Sep 14 19:14:47 CDT 2004

If you are not getting good immuno results with formalin fixatives you 
could try:

1. heat-induced antigen retrieval of your formalin fixed tissues.
2. Fixing in formalin for 4 hours instead of a longer time.
3. Fixing in a fixative that does not cross-link proteins (formalin is a 
cross-linker) such as Carnoy, methacarn or alcohol with some acetic acid 

I would try the above first. Bear in mind that different antigens have 
different fixative requirements for good immunoreactivity.


wasielewski.reinhard.von <@t> mh-hannover.de wrote:

>Hi Histonetters,
>We are looking for the most convenient and morpholgical satisfying fixative for 
>mouse and rat tissue beside formaldehyde. We want to stain a wide range of 
>different immunomarkers but don' t like formalin (therefore) too much. Frozen 
>sections are too bad in morphology.
>Please send me any suggestions !
>Many thanks in advance,
>PD Dr. med. Reinhard von Wasielewski
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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