[Histonet] Perferred fixative for animals

[Charles Scouten]

It might not be the formaldehyde.  Your freezing process sounds like a more likely culprit.  Read the following article:

http://www.myneurolab.com/neuronews/default.asp?AID=63&issueid=18&sitebannerid=&stylesheetid=

Use formaldehyde freshly made with paraformaldehyde in buffer (formalin bought as such degrades to include acids and acetone in it), include glutaraldehype.  Recipes may be seen at many places, including the manual for the Perfusion One. 
http://www.myneurolab.com/global/manuals/Perfusion%20One.pdf

The faster the fixative gets there after start of perfusion (I assume you are using perfusion) the better.  The Perfusion One can help with that.

http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21

Do not overfix.  Do not store in fixative for over a day.

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wasielewski.reinhard.von <@t> mh-hannover.de
Sent: Tuesday, September 14, 2004 7:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Perferred fixative for animals

Hi Histonetters,
We are looking for the most convenient and morpholgical satisfying fixative for mouse and rat tissue beside formaldehyde. We want to stain a wide range of different immunomarkers but don' t like formalin (therefore) too much. Frozen sections are too bad in morphology.
Please send me any suggestions !

Many thanks in advance,
Reinhard.

 

PD Dr. med. Reinhard von Wasielewski


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