[Histonet] vascular graft_sectioning difficulty
raghul <@t> sctimst.ker.nic.in
Tue Sep 14 06:26:23 CDT 2004
We cut 5 µm thin sections of paraffin embedded vascular graft(Dacron) in
a rotary microtome with disposable blades but the senior tech tells that
the tissue sections are crumbling and forces us to go for thick sections
around 10µm but again not with much success.
We can understand that there is not enough tissue adhereing to the
graft and we are literally cutting the graft. But did anyone had such
problems sectioning vascular grafts. Any suggestions?
Histopathology- implant biology division
Srichitra tirunal institute of medical sciences and technology
Trivandrum -695 012
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