[Histonet] vascular graft_sectioning difficulty

[Dr. Raghul]

hello members,

We cut 5 µm thin sections of paraffin embedded vascular graft(Dacron) in 
a rotary microtome with disposable blades but the senior tech tells that 
the tissue sections are crumbling and forces us to go for thick sections 
around 10µm but again not with much success.

 We can understand that there is not enough tissue adhereing to the 
graft and we are literally cutting the graft. But did anyone had such 
problems sectioning vascular grafts. Any suggestions? 


regards ,

Raghul J
scientist 
Histopathology- implant biology division
Srichitra tirunal institute of medical sciences and technology
Trivandrum -695 012
Kerala, India