[Histonet] Re: chromogen
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Sun Sep 12 08:32:28 CDT 2004
Gayle is completely right about the Permanent Red as Alk phos chromogen. I regard it as crisp as DAB, perhaps even better. If you wish to stick to HRP, indeed Vector Nova Red is a very good choice too. I would add that this substrate needs to be mounted fully organic, being an advantage over AEC. There is also an HRP chromogen called Romulin Red from BioCare. The colored reaction product appears to be identical to Vector Nova Red and perhaps they are chemically identical.
Hope this addition is helpful,
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
>From Gayle Callis <gcallis <@t> montana.edu>
Date Wed, 08 Sep 2004 13:29:26 -0600
To "Patsy Ruegg" <pruegg <@t> ihctech.net>, Histonet <@t> lists.utsouthwestern.edu
Subject Re: [Histonet] chromogen
DAKO has Permanent Red for alk phos IHC. Chris van der Loos indicated DAKO
product was very sensitive, and it also fluoresces. Vector red is AP and
also fluoresces. Have not tried Vector Red to compare with primary antibody
concentrations. These reds are more on dark pink red side, rather than
orangish red of AEC, or Brick red of NOVA Red.
NOVA Red from Vector, but it is a brick red!! Darker than AEC.
You can mount AEC from water, using Crystal mount, let this dry and then
add a permanent mounting media.
At 11:39 AM 9/8/2004, you wrote:
>What is a good substitute for DAB using HRP, my investigator wants
>prettier red or green colors for a poster, something like he gets with
>FITC, but I don't want to do flourescence? I know I could use AEC but I
>would prefer something more permanent. Didn't Kirkgaurd and Perry come
>out with a permanent red chromogen for HRP?
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