[Histonet] Re: chromogen

[C.M. van der Loos]

Dear Patsy,
Gayle is completely right about the Permanent Red as Alk phos chromogen. I regard it as crisp as DAB, perhaps even better. If you wish to stick to HRP, indeed Vector Nova Red is a very good choice too. I would add that this substrate needs to be mounted fully organic, being an advantage over AEC. There is also an HRP chromogen called Romulin Red from BioCare. The colored reaction product appears to be identical to Vector Nova Red and perhaps they are chemically identical.

Hope this addition is helpful,
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands


----- Original Message ----- 
>From  Gayle Callis <gcallis <@t> montana.edu> 
Date  Wed, 08 Sep 2004 13:29:26 -0600 
To  "Patsy Ruegg" <pruegg <@t> ihctech.net>, Histonet <@t> lists.utsouthwestern.edu 
Subject  Re: [Histonet] chromogen 
DAKO has Permanent Red for alk phos IHC.  Chris van der Loos indicated DAKO 
product was very sensitive, and it also fluoresces.  Vector red is AP and 
also fluoresces. Have not tried Vector Red to compare with primary antibody 
concentrations.  These reds are more on dark pink red side, rather than 
orangish red of AEC, or Brick red of NOVA Red.

NOVA Red from Vector, but it is a brick red!!  Darker than AEC.

You can mount AEC from water, using Crystal mount, let this dry and then 
add a permanent mounting media.

At 11:39 AM 9/8/2004, you wrote:
>What is a good substitute for DAB using HRP, my investigator wants
>prettier red or green colors for a poster, something like he gets with
>FITC, but I don't want to do flourescence?  I know I could use AEC but I
>would prefer something more permanent.  Didn't Kirkgaurd and Perry come
>out with a permanent red chromogen for HRP?
>Patsy
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610