[Histonet] Annette, synaptophysin

[Joyce Cline]

     I use a steamer (Black & Decker) for retrieval. I put the slides
into Cell Marque's Trilogy and steam for 20 min. Take the slides out  of
the steamer and let cool for 10 min. Place slides into APK wash
(ventana) for 5 min. I incubate for 32 min. No amplification or
blocking. I have no problem with light staining.



***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.