[Histonet] Land snail dissection.

Wed Sep 8 13:09:21 CDT 2004

Out of curiosity - is the shell made of calcium?  I'm asking because I 
really don't know - not a trick question?  Isn't a snail out of it's shell 
just a slug?
(Now THAT is a joke.)

Jackie O'

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotherapeutics
847.938.4919
Fax 847.938.3266

Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/08/2004 11:56 AM

        To:     histonet <@t> pathology.swmed.edu
        cc: 
        Subject:        Re: RE: [Histonet] Land snail dissection.

If the snail is small I recomend you to fix the whole snail and after 
fixation, decalcify it with 10 % EDTA. then process and include the whole 
snail. Hope this help

El dia 08/09/2004 18:23 usted envio el siguiente mensaje:

>Date: 8 de Septiembre de 2004 18:23:01

>From: "Smith, Allen" <asmith <@t> mail.barry.edu> 

>Subject: RE: [Histonet] Land snail dissection. 

>To: gcallis <@t> montana.edu, histonet <@t> pathology.swmed.edu

>

> Many centuries ago, I forced a snail out of its shell by shredding a 
pack of

> cigarettes into a pint of water and dropping the snail into it.

> Borradaile's THE INVERTEBRATA has instructions for dissecting the 
European

> garden snail Helix pomatia (pp. 604-610 in the 4th edition).  The book 
is

> out of print, but available used.

> 

> Allen A. Smith, Ph.D.

> Professor of Anatomy

> Barry University

> School of Graduate Medical Sciences

>             Podiatric Medicine and Surgery

> Miami Shores, Florida

> 

> 

> -----Original Message-----

> From: histonet-bounces <@t> lists.utsouthwestern.edu

> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle 
Callis

> Sent: Wednesday, September 08, 2004 10:56 AM

> To: andromeda_tm; Histonet <@t> lists.utsouthwestern.edu

> Subject: [Histonet] Land snail dissection.

> 

> 

> 

> Becaue of the hard calcified shell, dissection was still difficult.  The 

> snail didn't always relax all the way out of the shell.  I think we also 

> used another way to relax snail, but do not recall what was used (many 

> years ago!), as long as your method works, use it.

> 

> Since we could never get a snail totally out of shell (they don't give 
it 

> up easily!), we fixed snails whole, then decalcified them with shells 

> intact, and processed them into paraffin.  There is a "tooth" of some 
sort, 

> I do not recall what my snail experts called it, but it can create 
problems 

> during sectioning.  It was very hard and caused section damage.  There 
is 

> always a possiblity that after you decalcify the shell, you can remove 
it 

> very carefully to reach soft body parts.  We had wonderful sections with 

> thin shell intact - a total histological preparation.

> 

> We decalcified in 10 to 15% formic acid after the snail was totally 
fixed.

> 

>    At 04:48 AM 9/8/2004, you wrote:

> >Torino 08 September 2004

> >(ITALY)

> >

> >

> >Hi all,

> >

> >I am an amateur naturalist.

> >I like to study the histology of animal tissues by an optical 
microscope 

> >in transmitted light.

> >I am interested to land (terrestrial) snails.

> >I know for relaxing the snail to use a solution of 50mM of MgCl2 by an 

> >injection (2 ml.) into the foot.

> >Could someone give me some detailed information how to proceed to the 

> >snail dissection?

> >Thank you.

> >With my Best Regards,

> >

> >Massimo

> 

> Gayle Callis

> MT,HT,HTL(ASCP)

> Research Histopathology Supervisor

> Veterinary Molecular Biology

> Montana State University - Bozeman

> PO Box 173610

> Bozeman MT 59717-3610

> 406 994-6367 (lab with voice mail)

> 406 994-4303 (FAX)

> 

> 

> 

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> Barry University - Miami Shores, FL (http://www.barry.edu) 

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Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.

email: jluis.palazon <@t> icman.csic.es

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