[Histonet] Land snail dissection.

Wed Sep 8 11:56:54 CDT 2004

If the snail is small I recomend you to fix the whole snail and after fixation, decalcify it with 10 % EDTA. then process and include the whole snail. Hope this help

El dia 08/09/2004 18:23 usted envio el siguiente mensaje:
>Date: 8 de Septiembre de 2004 18:23:01
>From: "Smith, Allen" <asmith <@t> mail.barry.edu> 
>Subject: RE: [Histonet] Land snail dissection. 
>To: gcallis <@t> montana.edu, histonet <@t> pathology.swmed.edu
>
> Many centuries ago, I forced a snail out of its shell by shredding a pack of
> cigarettes into a pint of water and dropping the snail into it.
> Borradaile's THE INVERTEBRATA has instructions for dissecting the European
> garden snail Helix pomatia (pp. 604-610 in the 4th edition).  The book is
> out of print, but available used.
> 
> Allen A. Smith, Ph.D.
> Professor of Anatomy
> Barry University
> School of Graduate Medical Sciences
>             Podiatric Medicine and Surgery
> Miami Shores, Florida
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
> Sent: Wednesday, September 08, 2004 10:56 AM
> To: andromeda_tm; Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Land snail dissection.
> 
> 
> 
> Becaue of the hard calcified shell, dissection was still difficult.  The 
> snail didn't always relax all the way out of the shell.  I think we also 
> used another way to relax snail, but do not recall what was used (many 
> years ago!), as long as your method works, use it.
> 
> Since we could never get a snail totally out of shell (they don't give it 
> up easily!), we fixed snails whole, then decalcified them with shells 
> intact, and processed them into paraffin.  There is a "tooth" of some sort, 
> I do not recall what my snail experts called it, but it can create problems 
> during sectioning.  It was very hard and caused section damage.  There is 
> always a possiblity that after you decalcify the shell, you can remove it 
> very carefully to reach soft body parts.  We had wonderful sections with 
> thin shell intact - a total histological preparation.
> 
> We decalcified in 10 to 15% formic acid after the snail was totally fixed.
> 
>    At 04:48 AM 9/8/2004, you wrote:
> >Torino 08 September 2004
> >(ITALY)
> >
> >
> >Hi all,
> >
> >I am an amateur naturalist.
> >I like to study the histology of animal tissues by an optical microscope 
> >in transmitted light.
> >I am interested to land (terrestrial) snails.
> >I know for relaxing the snail to use a solution of 50mM of MgCl2 by an 
> >injection (2 ml.) into the foot.
> >Could someone give me some detailed information how to proceed to the 
> >snail dissection?
> >Thank you.
> >With my Best Regards,
> >
> >Massimo
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
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Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon <@t> icman.csic.es