[Histonet] p53 staining
settembr <@t> umdnj.edu
Tue Sep 7 13:37:45 CDT 2004
I use p53 on human tissue.
The antibody is from DakoCytomation used at a 1 :150 dilution with a
colon cancer as a control.
I use DakoCytomations Target Retrieval Solution in a steamer for 40
Antibody is incubated at Room Temperature for 15 minutes then Dako's
LSAB2 kit for detection.
Hope this helps.
University Hospital - UMDNJ
>>> Laurie Reilly <laurie.reilly <@t> jcu.edu.au> 9/6/2004 8:10:30 PM >>>
Does anyone have a protocol for doing p53 staining on Formalin Fixed
Paraffin Embedded tissues.
We are trying various methods of antigen retrieval and incubation times
canine mammary tumour, and hope to be able to apply the technique to
mast cell tumours.
Thanks for your consideration. Regards, Laurie.
Mr.Laurie Reilly Ph 07
Physiology & Pharmacology Fax 07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
4811 laurie.reilly <@t> jcu.edu.au
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