[Histonet] alkaline phosphatase staining

Fri Sep 3 17:06:30 CDT 2004

the enzyme alk.phos. stain for osteoblasts will not work on decalcified ffpe tissues in my experience.  it will work limitedly on bone processed into GMA without decal and cold methanol fixed.  I hear that there are antibodies to alk.phos. but I have not used them yet, has anyone else?
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Garlits,
John
Sent: Monday, August 30, 2004 11:04 AM
To: HistoNet Server
Subject: [Histonet] alkaline phosphatase staining

Hello,

I am interested in doing some alkaline phosphatase staining in decalcified, paraffin-fixed mouse long bone sections.  Does anyone have any experience with alk phos staining, who could recommend a protocol or even a commercial kit?

Thank you!

John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176

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