I've always used warm water when staining H&E on frozens. I've never had a problem with them floating off. I do use polylysine slides though. Linda Linda Blazek, HT (ASCP) Department of Pathology Children's Medical Center 1 Children's Plaza Dayton, Ohio 45404 (937) 641-3358 fax (937)641-5482 blazekl <@t> childrensdayton.org