[Histonet] RE: DAKO or VECTOR

[Edmondson David (RBV) NHS Christie Tr]

Think we have tried theirs, but ages ago and no advantage found.  so we kept
with what we knew best.     
Enjoy the weekend  
Dave

I hear that there is an archeological dig on nearby . And open day over the
weekend.   So I shall maybe have a look for histological remains. ( Did they
not use dyes and pigments back then.)    www.marple-uk.com

-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu]
Sent: 03 September 2004 15:15
To: Edmondson David (RBV) NHS Christie Tr
Subject: RE: DAKO or VECTOR


Thank you, I am better informed now.  Has this worked as well as VECTORS 
ABC kit?  or have you tried the latter?

Gayle Callis

At 03:39 AM 9/3/2004, you wrote:
>K0377,  is a kit where a drop of HRP and a drop of Streptavidin get mixed
>together in buffer,   The data sheet that I have from 1997 makes no mention
>of lifespan, now that I look
>Dave
>
>-----Original Message-----
>From: Gayle Callis [mailto:gcallis <@t> montana.edu]
>Sent: 02 September 2004 20:57
>To: Edmondson David (RBV) NHS Christie Tr
>Subject: DAKO or VECTOR
>
>
>Does DAKO have an avidin/bioin complex kit, I thought they has Labelled
>Strepavidin aka LSAB?  The ABC kit I am aware of is VECTOR's.
>
>At 10:47 AM 9/2/2004, you wrote:
> >To Cao Xudong/co Histonet
> >The DAKO ABC kit we used after several days, I thought they suggested a
>week
> >by way of a use-by time.  But that is a few years back now.
> >
> >Dave
> >Christie
> >Manchester UK
> >-----Original Message-----
> >From: John C. Dennis [mailto:dennijc <@t> vetmed.auburn.edu]
> >Sent: 02 September 2004 15:54
> >To: Cao, Xudong
> >Cc: histonet <@t> lists.utsouthwestern.edu
> >Subject: Re: [Histonet] vector ABC
> >
> >
> >
> >The Vector tech folk once told me that they'd used it up to several hours
> >(8-12).  I've kept it up to 4 hours but after that I dump it.
> >
> >John Carroll Dennis
> >Anatomy, Physiology, and Pharmacology
> >109 Greene Hall
> >Auburn University, AL  36849
> >
> >
> >On Wed, 1 Sep 2004, Cao, Xudong wrote:
> >
> > > Dear all,
> > >
> > > does anybody know how long a ready-to-use ABC solution is good for? I
am
> >thinking about re-use the mixed solution if it is good for more than 48
> >hours...
> > >
> > > thanks.
> > >
> > > Xudong Cao
> > > post-doc fellow
> > > Brown University
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> >
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>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)