[Histonet] Pre-cut controls

Richard Cartun Rcartun <@t> harthosp.org
Fri Sep 3 11:10:10 CDT 2004

We pre-cut all of our control tissue slides and store them at RT.  We
cut tonsil every week.  Most others are used up in a month's time, but
some do sit for a year or longer.  Obviously, every laboratory is
different, but you should do a study to determine how long your control
slides remain reactive.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Fran Lemons" <flemons <@t> bhset.org> 09/03/04 10:15AM >>>
Does anyone know why IHC control slides cannot be cut ahead of time? 
I've done it at other facilities, but I have been told it is not
permissible here, without any explanation.  Any ideas?
Also, has anyone ever heard of refrigerating pre-cut IHC control
slides?  One person suggested to me that they stopped cutting them ahead
of time because they were getting condensation on them & the stains
wouldn't work.  Then she informed me that they were stored in the
fridge.  Seems to me the condensation would have formed upon removing
the slides from a cold environment to a room temp.
Your thoughts/theories are appreciated.
Fran Walker
Histology Technical Specialist
ETBH Knoxville

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