[Histonet] RE: DAKO or VECTOR

[Edmondson David (RBV) NHS Christie Tr]

K0377,  is a kit where a drop of HRP and a drop of Streptavidin get mixed
together in buffer,   The data sheet that I have from 1997 makes no mention
of lifespan, now that I look
Dave

-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu]
Sent: 02 September 2004 20:57
To: Edmondson David (RBV) NHS Christie Tr
Subject: DAKO or VECTOR


Does DAKO have an avidin/bioin complex kit, I thought they has Labelled 
Strepavidin aka LSAB?  The ABC kit I am aware of is VECTOR's.

At 10:47 AM 9/2/2004, you wrote:
>To Cao Xudong/co Histonet
>The DAKO ABC kit we used after several days, I thought they suggested a
week
>by way of a use-by time.  But that is a few years back now.
>
>Dave
>Christie
>Manchester UK
>-----Original Message-----
>From: John C. Dennis [mailto:dennijc <@t> vetmed.auburn.edu]
>Sent: 02 September 2004 15:54
>To: Cao, Xudong
>Cc: histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] vector ABC
>
>
>
>The Vector tech folk once told me that they'd used it up to several hours
>(8-12).  I've kept it up to 4 hours but after that I dump it.
>
>John Carroll Dennis
>Anatomy, Physiology, and Pharmacology
>109 Greene Hall
>Auburn University, AL  36849
>
>
>On Wed, 1 Sep 2004, Cao, Xudong wrote:
>
> > Dear all,
> >
> > does anybody know how long a ready-to-use ABC solution is good for? I am
>thinking about re-use the mixed solution if it is good for more than 48
>hours...
> >
> > thanks.
> >
> > Xudong Cao
> > post-doc fellow
> > Brown University
> >
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> >
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)