[Histonet] alkaline phosphatase staining

[pruegg <@t> ihctech.net]

the enzyme alk.phos. stain for osteoblasts will not work on decalcified ffpe
tissues in my experience.  it will work limitedly on bone processed into GMA
without decal and cold methanol fixed.  I hear that there are antibodies to
alk.phos. but I have not used them yet, has anyone else?
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Garlits,
John
Sent: Monday, August 30, 2004 11:04 AM
To: HistoNet Server
Subject: [Histonet] alkaline phosphatase staining


Hello,

I am interested in doing some alkaline phosphatase staining in decalcified,
paraffin-fixed mouse long bone sections.  Does anyone have any experience
with alk phos staining, who could recommend a protocol or even a commercial
kit?

Thank you!

John Garlits, M.S.
Senior Research Technician
Hematology Oncology Division
Experimental Hematology Department
St. Jude Children's Research Hospital
332 N Lauderdale
Memphis, TN 38108
Phone: 901-495-3961
Fax: 901-495-2176


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