[Histonet] Warthin Starey

[Mildred Fail]

Sarah,
      We use the following method with good results though the Steiner is used more often some of our pathologists prefer the Warthin-Starry.
Solutions needed
1% Silver Nitrate 
2% Silver Nitrate 
0.15% Hydroquinone
5% Gelatin (use gentle heat to mix) 
All of the above are  stored at 2-8 degrees Celsius for up to 6 months.
!. Preheat a coplin jar with 1% silver nitrate in a 54-60 degree water bath for 30 minutes. Place your gelatin bottle in the water bath at the same time.
 2. Deparaffinize and hydrate slides to distilled water
3. Place slides in the preheated 1% Silver Nitrate and incubate for 30 minutes in a 54-60 degree water bath. At the same time place a vial of 1.5 mls of 2% silver nitrate, a vial of 3.75 5% gelatin, and a vial of 2 mls of 0.15% hydroquinone into the 54-60 degree waterbath to preheat.for 30 minutes.
4. Prepare the developer solution by mixing the solutions in the following order. 1.5 mls of 2% silver Nitrate
          3.75mls of 5 % gelatin and 2.0 mls of 0.15% hydroquinone
5. Remove slides from heated silver (DO NOT RINSE) Place slides horizontally on a paper towel and cover with developer. Allow sections to develop until they are a light golden brown. Check microscopically for end point
6. Rinse thoroughly in hot tap water
7. Dehydrate, clear and mount
 Rena Fail
 Medical University of SC
 


>>> "Blachford, Sarah - Histopath Main" <Sarah.Blachford <@t> ngh.nhs.uk> 09/02/04 05:27AM >>>

Can anyone help us.	
	
Has anyone got a reliable warthin starey method.  We have tried many methods
but cannot get them to work.

Also what is the use of gelatin in the method


Sarah


Northampton General Hospital NHS Trust 
Cliftonville, Northampton NN1 5BD 

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