[Histonet] Histology Resin

Janina Beyer beyer_janina <@t> yahoo.com.au
Thu Sep 2 00:44:25 CDT 2004

Hi I am a newcomer and have just discovered Histonet.

I have started an honours thesis in Tasmania, Australia, and will be looking at sex differentiation in the common carp.

I have been told that paraffin is not the best embedding method for fish embryos and problems with sectioning the yolk part occurs. It was recommended to me to use methyl methacrylate monomer and Benzoyl peroxide as a medium. 

I was wondering if anyone had used this for embedding embryos, and weather it is recommended? I am also looking at undertaking some immunohistochemistry with these samples, will that be a problem?

I would be grateful for any advice, or even references that you can suggest that may be helpful.

Best wishes,


Janina Beyer

Honours Student

University of Tasmania

CSIRO Marine Research

Hobart, Tasmania

Scientists  were rated as great heretics by the church, but they were truly religious men  because of their faith in the orderliness of the universe. 
--Albert Einstein

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