[Histonet] re-staining of "exhumed" sections
daniel.eberhard <@t> uni-bielefeld.de
Wed Sep 1 12:00:04 CDT 2004
I would like to re-stain old pFA fixed sections, which developed high
auto-fluorescence levels after some month. Thus, I decoversliped them,
removed (washed) the mounting media and performed normal antibody
staining using sec HRP conj. AB and DAB.
Unfortunately, some AB which recognize their epitopes in "fresh" pFA
fixed sections do not work on the "exhumed" sections. I wonder if
someone can assist me in solving this problem. Might antigen retrieval
methods help in this case?
Any hints may be helpful, thanks in advance
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