[Histonet] CD4 and CD8 in paraffin?
gcallis <@t> montana.edu
Wed Sep 1 11:32:52 CDT 2004
Teri et al,
You did not mention the species. If the staining was on human duodenal
biopsies (tough thing to do on a mouse!) rather than mouse tissue then
these markers work. The Mouse remains a total bust! Boo Hoo! Must be the
critter aka species differences rather than living Down Under, eh!??
At 08:49 AM 9/1/2004, you wrote:
>In this latest issue of Applied Immunohistochemistry and Molecular
>Morphology (AIMM 2004; 12:198-204) is an article in which CD4 and CD8
>are used in paraffin sections with antigen unmasking. CD4 (clone 1F6,
>1:20 from Novocastra, Malvern, Australia) and CD8 (clone C8/144B, 1:200;
>Dako, Sydney, Australia) were done on routinely processed duodenal
>biopsies. Maybe we're in the wrong hemisphere and that explains why it
>doesn't work for us?
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>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, Missouri 64110
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