[Histonet] wax embedding of plant tissues with latex

[J. A. Kiernan]

In "Plant Microtechnique and Microscopy," p.43,
S.E.Ruzin says fixation in formaldehyde-acetic-alcohol
is good for preserving laticifers. I suspect that
this would dissolve out the latex before it can
coagulate. On p.70 Ruzin recommends methacrylate 
embedding, and on p.143 he says laticifers can be 
fluorescently stained, giving a ref to Bruni & 
Tosi 1980. Protoplasma 102:343-347. 

In "Botanical Microtechnique and Cytochemistry,"
Berlyn & Mischke give a very detailed account
of how to infiltrate and embed plant specimens
but there is no mention of latex.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Kushan U Tennakoon wrote:
> 
> Dear Histonetters,
> 
> We have encountered problems when   trying to embed Euphorbia root tissues
> (that have  a lot of latex) in paraffin wax.
>  Can you please advice me / give some fine points to ensure the optimal
> penetration of paraffin wax into the plant tissues that have a lot of milky
> latex.
> 
> Sincerely,
> Kushan Tennakoon
> ktennako <@t> odu.edu
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet