[Histonet] wax embedding of plant tissues with latex

J. A. Kiernan jkiernan <@t> uwo.ca
Thu Oct 28 14:21:31 CDT 2004

In "Plant Microtechnique and Microscopy," p.43,
S.E.Ruzin says fixation in formaldehyde-acetic-alcohol
is good for preserving laticifers. I suspect that
this would dissolve out the latex before it can
coagulate. On p.70 Ruzin recommends methacrylate 
embedding, and on p.143 he says laticifers can be 
fluorescently stained, giving a ref to Bruni & 
Tosi 1980. Protoplasma 102:343-347. 

In "Botanical Microtechnique and Cytochemistry,"
Berlyn & Mischke give a very detailed account
of how to infiltrate and embed plant specimens
but there is no mention of latex.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Kushan U Tennakoon wrote:
> Dear Histonetters,
> We have encountered problems when   trying to embed Euphorbia root tissues
> (that have  a lot of latex) in paraffin wax.
>  Can you please advice me / give some fine points to ensure the optimal
> penetration of paraffin wax into the plant tissues that have a lot of milky
> latex.
> Sincerely,
> Kushan Tennakoon
> ktennako <@t> odu.edu
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