[Histonet] (no subject)

[Rocan]

I would suggest using labeling kits for your antibodies.  Molecular 
Probes has some that are very easy to use. You could directly label 
each antibody with a different alexa dye and have the result you want.  
This may cost a bit but you can label several antibodies even make your 
own secondaries for other applications. I believe the kist are called 
Zenon.
Good luck
Rocio
-----
Dr.Rocio Sierra-Honigmann
Director
Engineered Wound Repair Laboratory
Cedars Sinai Research Institute
Davis 1091
310-423-1882
Honigmannr <@t> cshs.org	k
On Oct 27, 2004, at 9:00 AM, Anne-Sophie Martinez wrote:

> Hi everybody,
>
> I am trying to stain in IHC, 2 isoforms of a protein present in the 
> kidney
> tubules of some fish. I would like to know if these proteins 
> colocalise or
> not in the same tubules.
> I can't use any confocal microscopy because I use two specific 
> polyclonal
> antibodies, both raised in rabbit. My option is then to have serial
> sections that I will stain: one with AB1 with a 2dry AB-FITC and one 
> with
> AB2 with a 2dry AB-Cy3. But this procedure bring two problems: (i) even
> serial sections don't have exactly the same structure and (ii) it is
> difficult to localise the same tubules on two different slides. Hope 
> all of
> this is clear.
> Would somebody have a suggestion for a better procedure or how to 
> improve
> this one?
> Thanks.
>
> AS
>
>
> ----------------------------------------------
> Dr Anne-Sophie Martinez
> School of Biology,
> University of St. Andrews,
> Bute Medical Buildings,
> St. Andrews, KY16 9TS, UK.
>
> Tel +44 (0) 1334 463063/3365
> Fax +44 (0) 1334 463600
> ----------------------------------------------
>
>
>
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