[Histonet] CD15

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Oct 26 19:30:28 CDT 2004


CD15 antibodies a very finicky.
Being an IgM antibody, you might need to use a anti-mouse IgM after the
localisation antibody to improve the sensitivity. I have also found a
difference in clones with Leu-M1 from Becton-Dickinson better than Dako's

Often overnight incubation with the Dako antibody will also improve the

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1 <@t> FAIRVIEW.ORG] 
Sent: Tuesday, 26 October 2004 11:14 PM
To: histonet <@t> pathology.swmed.EDU
Subject: [Histonet] CD15

Hi Histonetters,
We have recently had problems where our CD15 is NOT staining in any of
the Hodgkins cases we had - although, the CD30 is staining quite well. 
Our control will stain well but we have had 3 patients who have stained
negative with our procedure and yet positive at another institution.
This has been on both bone marrow cores and nodes.
We currently use Ventana's pre-dilute (clone MMA) on our Benchmark.
Has this happened to anyone else - or do you have any suggestions?
Thanks in advance.
Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454
amarusk1 <@t> fairview.org

The information transmitted in this e-mail is intended only for the person
or entity to which it is addressed
and may contain confidential and/or privileged material, including
"protected health information."  If you are
not the intended recipient, you are hereby notified that any review,
retransmission, dissemination,
distribution, or copying of this message is strictly prohibited.  If you
have received this communication in
error, please destroy and delete this message from any computer and contact
us immediately by return e-mail.

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 

More information about the Histonet mailing list