[Histonet] Working with toenails

[Bauer, Karen]

Joe,

We have had many problems with our toenails and fingernails staying on our
slides.  Lately we have been having good luck with them.  We do not soak or
pretreat the nails before processing, but once they are embedded and then
faced into, we soak the block in soapy water. (A few drops of Dawn dish soap
in a weigh boat with about 10 to 20 cc of tap water)  Let it soak for a
while, cut a section and place it on a slide.  We have had good luck with
Erie Scientifics Superfrost Plus Gold slides.  So far the sections have
stayed on pretty well.  We also use regular charged slides with a bit of
Elmer's Glue smeared on them.  Put the section on and air dry for about 30
minutes.  This works well too, but there is background from the glue.  

Good Luck, I know they are a pain to keep on the slides.

Karen Bauer HT(ASCP)
Histology Supervisor
Luther Hospital
Eau Claire,  WI

-----Original Message-----
From: Joe Nocito [mailto:JNocito <@t> Pathreflab.com]
Sent: Tuesday, October 26, 2004 8:46 AM
To: Histonet
Subject: [Histonet] Working with toenails


Hey everyone,
short story first. When I was in high school, I was a running back in
football (American football, not soccer). I quit because the middle line
backers and defensive ends were twice my size and I was getting killed.
	Stay with me just a bit longer. I promise this will be worth it.
	I didn't realize that I could have been a place kicker or a punter.
I could
have been known as "Joe the Toe".
Now, we are becoming the south Texas lab known for toenails, but we are
having a problem with them staying on. We also perform a PAS/Fungus. We have
tried Nair and ammonia water, but we are still having problems. Our sales
manager is going to all the podiatrists in town and we are getting inundated
with toenails. Can anyone give me some suggestions?
	This isn't what I had in mind when I said I wanted to be known as
"Joe the
Toe".
	thanks in advance.


Joe  "The Toe" Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX


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