[Histonet] need protocol for EM

Baowei Peng pengbw <@t> sjtu.edu.cn
Mon Oct 25 10:05:17 CDT 2004

Thanks for all replied to my post. 
Indeed I have no idea about using EM to observe tissue sections. What I want to do is using EM to trace a particle which is belived to be taken up by macrophage/DC in lymph node, the tracer have been said can be observed under EM. So I need a protocol in which I can learn from tissue removal through all steps to embedding.
I only have a machine can cut sections to 5 micrometer thick. But maybe I can get these material include grids from EM technologist.


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