[Histonet] re: microwave retrieval

[Jan Shivers]

I previously used plastic coplin jars set into a tupperware-type square
dish, but also had occasional evaporating/boiling down of buffer and
subsequent uncovering/drying out of tissues (even though I'd check and add
to the buffer level after every 5 minutes).

Now I use Tissue-Tek plastic containers, the rectangular-shaped ones that
are used in the deparaffinization/rehydration Tissue-Tek system.  They hold
more buffer volume, and it seems that less buffer is evaporated/boiled off.
I still check buffer levels after every 5 minutes of microwaving, but have
never lost too much buffer volume since switching.  And, the added bonus is
that each container can hold up to 24 slides in a Tissue Tek plastic slide
rack.

Jan Shivers
Univ. of Minnesota