[Histonet] Sectionnong whole-body rats with a BRIGHT 8250 Cryostat

[Alan Bright]

Dear Marie-Helene,

		   I do hope you have not been suffering these problems for to long, as the solution is simple. The knife angles are you are trying to section with are much too shallow and the specimen is being compressed by the rear end of the facet. The 'D' profile knife should be increased by 10º to 20º and the Tungsten Carbide Tipped knife increased by 6º to 26º.  With these major changes to the knife angles you will find that the sharp edge of the knife will then be able to section, the correct sectioning temperature is -18 to -20ºC.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Marie-Helene.Lavergne <@t> mdsps.com [mailto:Marie-Helene.Lavergne <@t> mdsps.com] 
Sent: 20 October 2004 18:58
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sectionnong whole-body rats with a BRIGHT 8250 Cryostat



   Hi everyone,

   I'm  using  a  Bright  8250 cryostat to do rat whole-body sections for
   QWBA  analysis.  Since I started using the microtome, (even with blood
   standards  blocks  or  a rat carcass) sometimes (1 time/10), the knife
   appears  to slip on the block at the begginning of the block and start
   to  section  at  the  end.   I  tried two type of knife (D-profile and
   tungsten) and many angles (generaly 10 degree for the D-profile and 20
   for tungsten)

   I  tried  to  adjust the slide tensioners. I tried two types of CMC. I
   tried different temperature (-20 c, -25 c) and it still doesn't work.



   WHAT IS THE PROBLEM????

   

   Please, HELP ME, I'm trying to solve this problem since many months.



   Marie-Helene Lavergne

   Analyst DMPK

   MDS Pharma

   [1]marie-helene.lavergne <@t> mdsps.com

   

References

   1. file://localhost/tmp/mne@mdsps.com
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