[Histonet] Microwave retrieval

[Katri Tuomala]

Hi Ed,

My experience:
Microwave with coplin jars overboils and you need to add buffer periodically 
(drying of tissue can happen), hot spots in microwave often result in uneven 
staining.
We used microwavable pressurecooker (Dako) for many years and results 
improved, but we still had uneven staining occasionally.
Now we are using the Decloaker and results are more reproducible and a log 
of temperature/pressure can be kept. However, we did have to do some 
adjustments in primary antibody dilutions. Concentrations had to be 
increased with some, therefore costing more.
Steamer, I personally have not used, but my understanding is that 
reproducibility is pretty good, but the procedure takes a bit more time.
My ten cent's worth...

Katri

Katri Tuomala
Hamilton, Ontario, Canada



----- Original Message ----- 
From: "Harris, Ed" <Hared <@t> FGHI.COM>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 20, 2004 11:30 AM
Subject: [Histonet] Microwave retrieval


Can anyone give me the pro's and con's of steamer/pressure (Decloaker) vs.
microwave retrieval ?
Our Doc wants us to change from the Decloaker to microwave. Is a household
microwave adequate or do we need a lab grade unit?
Does the microwave save time and improve turn around time?

Thanks in advance
Ed Harris  HT ASCP MT ABB



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