[Histonet] Microwave retrieval
katri <@t> cogeco.ca
Wed Oct 20 17:11:24 CDT 2004
Microwave with coplin jars overboils and you need to add buffer periodically
(drying of tissue can happen), hot spots in microwave often result in uneven
We used microwavable pressurecooker (Dako) for many years and results
improved, but we still had uneven staining occasionally.
Now we are using the Decloaker and results are more reproducible and a log
of temperature/pressure can be kept. However, we did have to do some
adjustments in primary antibody dilutions. Concentrations had to be
increased with some, therefore costing more.
Steamer, I personally have not used, but my understanding is that
reproducibility is pretty good, but the procedure takes a bit more time.
My ten cent's worth...
Hamilton, Ontario, Canada
----- Original Message -----
From: "Harris, Ed" <Hared <@t> FGHI.COM>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 20, 2004 11:30 AM
Subject: [Histonet] Microwave retrieval
Can anyone give me the pro's and con's of steamer/pressure (Decloaker) vs.
microwave retrieval ?
Our Doc wants us to change from the Decloaker to microwave. Is a household
microwave adequate or do we need a lab grade unit?
Does the microwave save time and improve turn around time?
Thanks in advance
Ed Harris HT ASCP MT ABB
This E-mail message (including attachments, if any) is intended for the use
of the individual or entity to which it is addressed and may contain
information that is privileged, proprietary, confidential and exempt from
disclosure. If you are not the intended recipient, you are notified that
any dissemination, distribution or copying of this communication is strictly
prohibited. If you have received this communication in error, please notify
the sender and erase this E-mail message immediately.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet