[Histonet] Hydrophobic properties on red control box slides.

Wayne Holland wayneholland1959 <@t> msn.com
Wed Oct 20 11:00:29 CDT 2004

Let me make it clear that the manufacter is working closely with me to 
resolve this problem, they have offered much help in this matter. They are 
also examining the lots that were used. Maybe I thought I was crazy. Your 
input please!

>From: "Wayne Holland" <wayneholland1959 <@t> msn.com>
>To: Histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Hydrophobic properties on red control box slides.
>Date: Wed, 20 Oct 2004 11:44:16 -0400
>Has anyone experienced using an automated immuno system that maintains the 
>slides on a horizontal plain during the run, having possible hydrophobic 
>properties. This prevents the antibody and other reagents from being 
>dispersed evenly on the slide. The reagents on this system are dispensed in 
>the area of the control box (with the control box being painted on the 
>underside of the slide). However when these slide are shipped, they are 
>boxed back to front laying on one another of course, this causing the paint 
>properties to be transferred to the slide in front if it. I have found that 
>I have had to run the coated slides through Xylene and alcohol, then air 
>dry under a hood before we mount the tissue. Has anyone experienced this 
>being a problem? Once we started this additional step, all the problems of 
>the tissue in the control box staining and not the patient has been 
>eliminated. PLEASE HELP. The people that make the glass think I'm crazy.
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