[Histonet] Hydrophobic properties on red control box slides.
wayneholland1959 <@t> msn.com
Wed Oct 20 10:44:16 CDT 2004
Has anyone experienced using an automated immuno system that maintains the
slides on a horizontal plain during the run, having possible hydrophobic
properties. This prevents the antibody and other reagents from being
dispersed evenly on the slide. The reagents on this system are dispensed in
the area of the control box (with the control box being painted on the
underside of the slide). However when these slide are shipped, they are
boxed back to front laying on one another of course, this causing the paint
properties to be transferred to the slide in front if it. I have found that
I have had to run the coated slides through Xylene and alcohol, then air dry
under a hood before we mount the tissue. Has anyone experienced this being a
problem? Once we started this additional step, all the problems of the
tissue in the control box staining and not the patient has been eliminated.
PLEASE HELP. The people that make the glass think I'm crazy.
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