[Histonet] Eosin, phloxine and OG?

[Marshall Terry Dr, Consultant Histopathologist]

Come on John - you can't get away with half a story:-)
If H&E isn't a good routine stain, what is (and how many years to get used to it)?

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: John Kiernan [mailto:jkiernan <@t> uwo.ca]
Sent: 20 October 2004 06:26
To: WWmn916 <@t> aol.com
Cc: histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] Eosin, phloxine and OG?


If you look at your H&E slides between staining and 
clearing & coverslipping you will find that eosin Y 
(CI 45380) can impart more than 3 colours to RBC,
smooth muscle cytoplasm, other cytoplasms and
collagen. 

Adding phloxin or orange G to an eosin Y counterstain
may extend the spectrum, but don't expect clear-cut
results from slides stained by a machine. 

The H&E method needs human judgement and adjustments
before applying a coverslip. For this reason and
others H&E is not a good "routine" staining method.
It's all there in the peer-reviewed literature and
in most histotechnology textbooks less than 25
years old. 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
> (for H+E staining)?
> The conversation came up recently to "amp" the three shades of red in H+E
> staining.  It was the first time I'd ever heard of adding Orange G into
> eosin/phloxing solution. Are there any labs doing this?
> 
> Thanks:-)
> Deb
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