[Histonet] Acid cleaning slides

[Greg Dobbin]

Hi Janice,
I certainly may be corrected on this, but I don't believe your "Plus" 
slides will be "Plus" slides anymore if you acid wash them. I would 
think that an acid wash would remove the positive charge from the 
slides.

A few years ago, when I was coating/charging my own slides, I 
would acid wash and alcohol dry regular glass slides prior to 
treatment with Poly-L Lysine or Silane (APES). The acid wash 
helped remove any oily film that may have accumulated on the 
slides in storage, that might have interfered with proper coating 
of the Poly-L or APES. 
Greg

Date sent:      	Tue, 19 Oct 2004 09:52:31 -0500
From:           	"Janice A Mahoney" <jmahoney <@t> alegent.org>
To:             	<histonet <@t> lists.utsouthwestern.edu>
Subject:        	[Histonet] Acid cleaning slides

> Hello,
> I would like to know if anyone has had problems with attempting to acid clean plus slides.  Does this process affect the adhesion of cells to the slide.  We have some background staining on our HPV thin prep slides and are trying to eliminate the background.
> Thanks,
> Jan Mahoney
> Omaha NE
> 
> 
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada,  C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
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Happiness is a journey, not a destination.