[Histonet] Dog antibody search

lfit4832 <@t> mail.usyd.edu.au lfit4832 <@t> mail.usyd.edu.au
Tue Oct 19 07:34:36 CDT 2004

Hi Jackie,

The only Ki-67 antibody successfully used in canine tissue (in the
literature I have read anyway) is the Immunotech MIB-1 clone. Immunotech
antibodies are now distributed by Beckman Coulter, and are indicated by
(IM) in front of the catalogue number - think the catalogue number for the
Ki67 (MIB-1) monoclonal is IM1316.

I have 2ml of it in the fridge and I am planning to run it on a bunch of
canien tumours over the summer.

Here are a few references:

Geraldes, M., F. Gartner, et al. (2000). "Immunohistochemical study of
hormonal receptors and cell proliferation in normal canine mammary glands
and spontaneous mammary tumours." Veterinary Record 146(14): 403-6.

Laprie, C., J. Abadie, et al. (2001). "MIB-1 immunoreactivity correlates
with biologic behaviour in canine cutaneous melanoma." Veterinary
Dermatology 12(3): 139-47.

Madewell, B. R. (2001). "Cellular proliferation in tumors: a review of
methods, interpretation, and clinical applications." Journal of Veterinary
Internal Medicine 15(4): 334-40.

Millanta, F., F. Fratini, et al. (2002). "Proliferation activity in oral
and cutaneous canine melanocytic tumours: correlation with histological
parameters, location, and clinical behaviour." Research in Veterinary
Science 73(1): 45-51.

Perez Alenza, M. D., L. Pena, et al. (2000). "Factors influencing the
incidence and prognosis of canine mammary tumours.[see comment]." Journal
of Small Animal Practice 41(7): 287-91.

Phillips, B. S. (2000). "Apoptotic and proliferation indexes in canine
lymphoma." Journal of Veterinary Diagnostic Investigation 12(2): 111-117.

Roels, S., K. Tilmant, et al. (1999). "PCNA and Ki67 proliferation markers
as criteria for prediction of clinical behaviour of melanocytic tumours in
cats and dogs." Journal of Comparative Pathology 121(1): 13-24.

Zacchetti, A., E. van Garderen, et al. (2003). "Validation of the use of
proliferation markers in canine neoplastic and non-neoplastic tissues:
comparison of KI-67 and proliferating cell nuclear antigen (PCNA)
expression versus in vivo bromodeoxyuridine labelling by
immunohistochemistry." APMIS 111(3): 430-8.



Quoting Jackie.O'Connor <@t> abbott.com:

> Hi guys -
> Can anyone steer me towards a supplier for Caspase-3, MPM-2, and Ki-67
> primary antibodies that will work for IHC on FFPE Dog??
> Thanks much!
> Jackie O'
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