[Histonet] Nuclear Bubbles

[Annette Hall]

Hi,

Over the past 6 months, our lab has struggled intermittently with an
artifact on our H&E slides. The nucleus will appear to have bubbled. This
seems to be most prevalent in prostate biopsies, but we have also seen it in
endometrial biopsies. We use a Leica Autostainer XL with Richard Allan
reagents. We have adjusted drying times and oven temps but we still continue
to occasionally see bubbles. Has anyone had this experience or know what is
causing this? Any suggestions would be greatly appreciated.

Thanks,
Annette J. Hall, MT
Micro/Histo/Cyto Supervisor
United Clinical Labs
205 Bluff St.
Dubuque, IA 52001
563.556.2010 x131