[Histonet] membranes

[Elizabeth Chlipala]

Patsy
 
We routinely process organotypic cultures on membranes, the ones that we
are most familiar with are from Mattek, (I think they are Millipore
MillicellT CM cell culture inserts) we process just like regular tissue
and our tissue processor has xylene on it.  We use a 8mm biopsy punch to
remove the culture and membrane from the culture insert, process it
between biospsy sponges and cut in half and embed each halve on end.
We have not experienced any problems with sectioning, occasionally there
might be some separation of the membrane from the tissue, but not that
often.  I'll send you some images.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
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University of Colorado
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Boulder, Colorado 80309