[Histonet] bone decalcification

[Jose Luis Palazon Fernandez]

Dear List-fellows

I want to thank all of you that answered to my query about quick decalcification.

best wishes

José Luis




El dia 12/10/2004 19:13 usted envio el siguiente mensaje:
>Date: 12 de Octubre de 2004 19:13:01
>From: "Patsy Ruegg" <pruegg <@t> ihctech.net> 
>Subject: RE: [Histonet] bone decalcification 
>To: jluis.palazon <@t> icman.csic.es, histonet <@t> lists.utsouthwestern.edu
>
> Jose,
> If you are thinking of doing IHC Formic Acid is a good decal to use
> (5%).  Decal Chemicals sells a formic acid product called Immunocal.
> There are other quicker products that I believe use Nitric acids but IHC
> is better with the Formic in my hands.
> Patsy
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jose
> Luis Palazon Fernandez
> Sent: Tuesday, October 12, 2004 3:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] bone decalcification
> 
> 
> Dear List-members
> 
> 
> 
> I have to dacalcify some fish vertebrae in order to see the vertebral
> articulation and intervertebral discs, and have to use a quieck method.
> I usually decalcify with EDTA but this method is very slow. I would
> appreciate your advise. 
> 
> 
> 
> Thanks in advance!
> 
> 
> 
> José Luis
> 
> Universidad de Oriente-Isla Margarita-Venezuela
> 
> actualmente en: Instituto de Ciencias Marinas de Andalucia
> 
> Puerto Real, Cádiz, España.
> 
> email: jluis.palazon <@t> icman.csic.es
> 
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Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon <@t> icman.csic.es