[Histonet] lypmh node sections folding and falling off of immuno slides

Robyn Vazquez vazquezr <@t> ohsu.edu
Tue Oct 12 13:03:50 CDT 2004


Travis,
Try incubating overnight in a 37c oven.  That might help.  Are you
cutting them at 3 microns?
 
Robyn 
OHSU
Portland, OR

>>> "Travis Troyer" <ttroyer <@t> pcllab.com> 10/12/2004 9:52:52 AM >>>

I was wondering if there were any tricks to getting lymph nodes to
remain flat and intact in order to do immuno stains.  I have a problem
with the tissue falling off or folding after the immuno run.    Any
thoughts or suggestions would be much appreciated.
Thanks,
Travis Troyer
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