[Histonet] Calcein Bone Labeling

Pat Flannery pjfnefro <@t> duke.edu
Mon Oct 11 15:30:35 CDT 2004


Try dissolving the calcein at 1 mg/ml in 2% Sodium Bicarbonate with 0.9% NaCl.  The bicarb makes up for the pH of the calcein. If you want to see the pH difference, try dissolving the calcein in the NaCl first then add the bicarb and you'll see a dramatic color change from dull orange-yellow to brilliant glowing yellow.

Hope this helps.


-Patrick J. (Pat) Flannery
 Division of Nephrology (that's kidneys to you)
 Box 3014 (that's NOT "PO" just "Box")
 Duke University Medical Center
 Durham, NC  27710
 E-mail: pjfnefro <@t> duke.edu (preferred)
         FLANN002 <@t> MC.DUKE.EDU (also works)
Voice:   (919)660-6863
Fax:     (919)684-2929

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