[Histonet] bone marrow for immunostaining - how?

[Jackie.O'Connor <@t> abbott.com]

Are you collecting human or animal bone marrow?  I am able to do CD45 
immunostaining on mouse bone marrow by harvesting the entire femur in 10% 
formalin.
The femur is then decalcified in 5% formic acid, and processed for 
paraffin embedding.  Immunostaining is performed routinely, with Citrate 
buffer antigen retrieval.
Jackie O'Connor.




"Julia Edgar" <je22r <@t> udcf.gla.ac.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/11/2004 03:01 AM

 
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        cc: 
        Subject:        [Histonet] bone marrow for immunostaining - how?


Dear All
I need to collect some bone marrow to test out an antibody to CD45. Can 
you please tell me how to go about it and how to store the material once 
collected.
Thank you in advance
Julia
Julia Edgar, BSc (Hons), PhD
University of Glasgow Veterinary School
Glasgow G61 1QH
e-mail: je22r <@t> udcf.gla.ac.uk
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