Fwd: Re: [Histonet] mouse brains

[Atoska S. Gentry]

>Date: Thu, 07 Oct 2004 09:43:12 -0500
>To: meyenhmf <@t> umdnj.edu
>From: "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>
>Subject: Re: [Histonet] mouse brains
>
>
>
>Thanks for your inquiry. Acquiring fairly decent sections is involving a 
>lot more trial and error than previous sections processed & done a few 
>months back using the same procedure. Pointers & input will be much 
>appreciated. Atoska
>
>At 02:54 PM 10/6/2004, you wrote:
>>What IS the problem?/
>>
>>Quoting "Atoska S. Gentry" <gentras <@t> vetmed.auburn.edu>:
>>
>> >
>> > Hello, those of you processing & sectioning coronal sections of mouse
>> > brain; from you experience can prolonged fixation in 4%PFA  be the
>> > culprit
>> > of sectioning problems? What is the maximum fixation time period the
>> > brain
>> > should held in PFA before processing & sectioning? Thanks! Atoska
>> >
>> >
>> > Atoska S. Gentry B.S., HT(ASCP)
>> > Research Assistant III
>> > Scott-Ritchey Research Center
>> > College of Veterinary Medicine
>> > Auburn University, AL  36849
>> > Phone# (334)844-5579  Fax# (334)844-5850
>> >
>> >
>> > _______________________________________________
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>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>>
>>
>>--