[Histonet] GI bx

[Pamela Marcum]

Actually you may need to check that even more fully.  The SDA 3 alcohols we
use as reagent alcohol to avoid tax and the ATF contains both isopropanol
and methanol at 3% to 5% of each type depending on the supplier and the
license they have for manufacture.  In a university or non-taxable
institution you can afford the really good pure stuff while the tax often
means the rest of us have to find something cheaper and carefully revamp our
processing and thinking.  I know there are other netters who can explain
this in far more detail however this is the basic.

Thanks,

Pam Marcum 



> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Joanne
> Mauger
> Sent: Thursday, October 07, 2004 8:28 AM
> To: mbecker <@t> pathlabinc.com; histonet <@t> pathology.swmed.edu;
> JWEEMS <@t> sjha.org
> Subject: RE: [Histonet] GI bx
>
>
> We use 95% ethanol without methanol added, but our absolute does
> contain a small amount. For us, changing the 95% made all the
> difference. we had switched companies, and didn't realize that
> most ethanols had ethanol in them. Our bxs got real crunchy. When
> we read the 95 ingredients more carefully, we found the culprit,
> and when we replaced it with the non meth. 95 the bxs were
> gorgeous again! Our pharmacy orders the 95% from Aaper in
> Shelbyville,Kentucky.
> jo
>
> >>> "Weems, Joyce" <JWEEMS <@t> sjha.org> 10/07/04 08:07AM >>>
> This is the very first time I recall hearing that methanol might
> make them crispy! What kind of alcohols do you use?
>
> Thanks, j
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 404-851-7376 - Phone
> 404-851-7831 - Fax
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Joanne
> Mauger
> Sent: Thursday, October 07, 2004 7:48 AM
> To: mbecker <@t> pathlabinc.com; histonet <@t> pathology.swmed.edu
> Subject: Re: [Histonet] GI bx
>
>
> Michele,
> Could be crush artifact from clinicians or the gross bench. We
> use a shorter processing program for GI bxs. Make sure your 95%
> alc doesn't have methanol in it. This makes bxs very crispy(dries
> out too much)Drove us crazy too.
> Jo
>
> >>> "Michele Becker" <mbecker <@t> pathlabinc.com> 10/06/04 04:21PM >>>
> Please help....................  We are having problems with blurring and
> distorted nuclei on some of our GI biopsies. This has been
> happening on and
> off for the past few months.  It is not every day and not all the GI bx on
> any given day. We have ruled out staining and processing. I
> really think it
> is something happening to the bxs before we even receive them but want to
> make sure we have not left any stones unturned on our end first.  Any
> suggestions?
>
> Thank-you.
>
> Michele
>
>
>
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