[Histonet] mouse brains

[Charles Scouten]

Are you perfusing, or just extracting and dropping in PFA?  Sorry, what is PFA?  Perfusion is necessary for good fixation, and is easy to do.   Overfixation can lead to loss of immuno or other reactivity, but can only improve sectioning.  I would guess you are not fixing fast enough, your tissue is bad before fixative gets a chance to do its work.

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry
Sent: Wednesday, October 06, 2004 2:09 PM
To: Histonet
Subject: [Histonet] mouse brains


Hello, those of you processing & sectioning coronal sections of mouse brain; from you experience can prolonged fixation in 4%PFA  be the culprit of sectioning problems? What is the maximum fixation time period the brain should held in PFA before processing & sectioning? Thanks! Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 


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