[Histonet] RE: Histonet Digest, Vol 11, Issue 7

Hopkins, Karen KMH.02 <@t> ex.uchs.org
Wed Oct 6 12:16:44 CDT 2004


Comment about thinprep filter....I was also curious if anyone has done any "validation" studies to compare using the blue non-gyn filter instead of the clear gyn filter for gyn specimens.  Apparantly the filter pores are smaller on the blue one so it should be okay- the problem is that the thinprep test for gyn's is an FDA approved test for use as the manufacturers instructions and to use the blue one would mean having to validate the results.  I was curious because the cost of the blue filters is much less than the clear ones.  Any thoughts?

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, October 06, 2004 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 11, Issue 7


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Today's Topics:

   1. Fisher 266mp tissue processor Err 03, any help (Ze Lu)
   2. RE: tissue processors (GUTIERREZ, JUAN)
   3. Re: mouse paws (jameel.syed <@t> abbott.com)
   4. (no subject) (Achstetter, Virginia A.)
   5. Mounting media/ethanol (Cindy DuBois)
   6. HPV Ventana Benchmark (Sanders, Julie, VHACIN)
   7. RE: mouse paws (Elizabeth Chlipala)
   8. SV: [Histonet] Mounting media to covberslip from ethano
      (Eva Alstr?mer)
   9. decalcified sections of bone (Nicole Hedgecock)
  10. Attn: Ann Featherstone, failed message delivery (Gayle Callis)
  11. tissue processing (Robyn Vazquez)
  12. RE: Tissuearrays (Thom Jensen)
  13. NADPH-TR protocol (Sylvia Poulos)
  14. More details: decalcified sections of bone (Nicole Hedgecock)
  15. orienting mouse embryos (lkbauer <@t> unmc.edu)
  16. RE: orienting mouse embryos (Bonner, Janet)
  17. Re: orienting mouse embryos (Laurie Reilly)
  18. Re: orienting mouse embryos (ajennings <@t> unmc.edu)
  19. Reusing the filter for ThinPrep for HPV (Gervaip <@t> aol.com)
  20. Bone Saw and Dust Extraction (Darren James)
  21. Re: DAB-colors... (SMITH,REBEKAH FELICIA)
  22. elastic fibers (Jose Luis Palazon Fernandez)
  23. processing question (Molinari, Betsy)
  24. RE: Reusing the filter for ThinPrep for HPV (Joe Nocito)
  25. Cassette Label System (Paula Lucas)
  26. Eye Protection Question (Jordan Brod)
  27. Re: Eye Protection Question (Jackie.O'Connor <@t> abbott.com)
  28. RE: Cassette Label System (Joe Nocito)
  29. RE: Eye Protection Question (Joe Nocito)
  30. micronucleus staining (Fawn Jones)


----------------------------------------------------------------------

Message: 1
Date: Tue, 5 Oct 2004 13:12:40 -0700
From: "Ze Lu" <lu_ze <@t> sbcglobal.net>
Subject: [Histonet] Fisher 266mp tissue processor Err 03, any help
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <027401c4ab17$aa907d50$1302a8c0 <@t> optimum2>
Content-Type: text/plain;	charset="iso-8859-1"

Hello, histonet friends,

We had a quite old Fisher 266mp tissue processor in our lab. Every time when we start the program cycle, it showed Err 03 on screen. only very low level of solution go into the process pot. From the manual, it is indicated as overflow. We are quite sure that we did not load a lot of into tank. We also check the vacuum ortice in the process pot, and it is not blocked. The rotation valve is aldo fine when we checked. Could it be a problem of vacuum pump or leaking in some tubing? We appreciate your help. 

Ze Lu
Optimum Therapeutics, LLC

------------------------------

Message: 2
Date: Tue, 5 Oct 2004 12:24:23 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] tissue processors
To: "Andres Kulla" <Andres.Kulla <@t> kliinikum.ee>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A61F23E937E4DA488E0C0F60093843D901E497BB <@t> ccetxm030.echristus.net>
Content-Type: text/plain;	charset="iso-8859-1"

Nothing beats the Tissue Teks.  They keep going and going and going.  Sorry but I can not say anything bad about equipment or companies anymore.  Too many damn lawyers here in the USofA.

Juan

-----Original Message-----
From: Andres Kulla [mailto:Andres.Kulla <@t> kliinikum.ee] 
Sent: Tuesday, October 05, 2004 1:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue processors

To the experts in automated tissue processing,
Could anybody share his/her experience with TBS (Triangle Biomedical Sciences) ATP1 tissue processor. Positive vs negative aspects in comparison with other devices eg Sakuras Tissue Tek VIP 500.
With best regards from 
Andres Kulla,
Estonia

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------------------------------

Message: 3
Date: Tue, 5 Oct 2004 13:29:01 -0400
From: jameel.syed <@t> abbott.com
Subject: [Histonet] Re: mouse paws
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFEEBC49B3.DB32476D-ON85256F24.005E35BD <@t> northamerica.intra.abbott.com>
	
Content-Type: text/plain; charset="iso-8859-1"

We process paws routinely.  We haven't felt the need to deglove the skin. 
However, we decal them briefly and trim the foot pad longitudinally on 
both sides.  After trimming we complete the decaling and process them with 
a program that is slightly longer paraffin infiltration.  Trimming the 
skin off greatly expedites decaling and improves infiltration.  In case of 
highly arthritic samples it makes them small enough to fit in a cassette 
for processing.

Good luck,
Jameel Syed, BS, HT/HTL, QIHC (ASCP)
Pathology Supervisor
Abbott BioResearch Center
100 Research Drive, Worcester, MA 01605
Tel: (508)849-2862
Fax: (508)793-4895






---------------------------------------------------------------------

Message: 1
Date: Tue, 05 Oct 2004 08:30:42 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] mouse paws
To: "joyce judge" <joycejudge259 <@t> hotmail.com>,
                 Histonet <@t> lists.utsouthwestern.edu
Message-ID:
 <6.0.0.22.1.20041005082945.01b0f408 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed

No we leave paws intact.  However, mouse paws are extremely dense even 
though tiny,  and after decalcification, we do a longer processing 
schedule.

At 05:15 AM 10/5/2004, you wrote:
>I would like too know if people that are processing mouse paws deglove or 

>process with the skin on.
>
>Joyce Judge
>Visen Medical
>jjudge <@t> visenmedical.com
>
>_________________________________________________________________
>Is your PC infected? Get a FREE online computer virus scan from McAfee® 
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>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

***************************************


------------------------------

Message: 4
Date: Tue, 5 Oct 2004 13:45:11 -0400
From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9C631520464F9E4BA5B111450F2A0AAD0BCAF3 <@t> lewis.afip.osd.mil>
Content-Type: text/plain;	charset="iso-8859-1"

Does anyone have a good protocol for cutting Microarray blocks?  I understand that there are different methods of sealing the block cores so they stay put when sectioning.

Ginny Achstetter HT (ASCP)
Armed Forces Institute of Pathology
Soft Tissue Pathology
6825 16th St. NW
Bldg 54 Rm. 3062
Washington, DC 20306
Fax: 202-782-9182
Phone: 202-782-1914



------------------------------

Message: 5
Date: Tue, 5 Oct 2004 10:53:58 -0700 (PDT)
From: Cindy DuBois <dpahisto <@t> yahoo.com>
Subject: [Histonet] Mounting media/ethanol
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20041005175358.18251.qmail <@t> web41303.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

We use Clearium from  Surgipath and coverslip out of Isopropyl alcohol.  We have been doing this for several years.  One drawback we have seen is if they dried to quickly (ie. put in oven for drying), we see lots of bubbles.  We just put the flats on top of the embedding center for about 15 minuts and they are dry enough for the docs.  Then when we get them back we let them sit at room temp overnight, then put in over (60 C) overnight.
Another drawback is when using it with any alcohol soluble stain (Mucicarmine, Gram, etc.) the stain will leach out over time.  On these slides, we merely coverslip out of xylene.
 
Hope this helps,
Cindy DuBois, HT ASCP
Delta Pathology
Stockton CA

		
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Yahoo! Mail Address AutoComplete - You start. We finish.

------------------------------

Message: 6
Date: Tue, 5 Oct 2004 12:53:26 -0500 
From: "Sanders, Julie, VHACIN" <Julie.Sanders <@t> med.va.gov>
Subject: [Histonet] HPV Ventana Benchmark
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<457381D92B01BD44B21CF37CC02EBDFD28E97A <@t> vhacinexc2.v10.med.va.gov>
Content-Type: text/plain;	charset="iso-8859-1"

Are you looking for a quote for the Benchmark itself, or the cost of doing
HPV's?
You can contact Ventana to have a sales rep visit your site if looking for
the machine.  If you want cost for doing HPV's, I could figure up what it is
costing us.
Julie
Julie Sanders, BA, HT(ASCP)
Supervisor, Anatomic Pathology
VAMC, Cincinnati, Oh.




------------------------------

Message: 7
Date: Tue, 5 Oct 2004 12:07:57 -0600
From: "Elizabeth Chlipala" <lizchlipala <@t> premierhistology.com>
Subject: RE: [Histonet] mouse paws
To: "'joyce judge'" <joycejudge259 <@t> hotmail.com>,
	<histonet <@t> pathology.swmed.edu>
Message-ID: <002601c4ab06$3e5ef320$76d48a80 <@t> AMY>
Content-Type: text/plain;	charset="US-ASCII"

We routinely process mouse paws for arthritis studies and we do not take
the skin off.  This way the specimens remain intact. I agree with Gayle
and that we also process on a longer processing cycle also.  There are
pictures on our web site of mouse paws and ankles from arthritis
studies, just go to the gallery.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joyce
judge
Sent: Tuesday, October 05, 2004 4:16 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] mouse paws

I would like too know if people that are processing mouse paws deglove
or 
process with the skin on.

Joyce Judge
Visen Medical
jjudge <@t> visenmedical.com

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------------------------------

Message: 8
Date: Tue, 5 Oct 2004 20:32:12 +0200
From: Eva Alstr?mer <eva.alstromer <@t> histolab.se>
Subject: SV: [Histonet] Mounting media to covberslip from ethano
To: "'Paul Bradbury'" <histology.bc <@t> shaw.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <001501c4ab09$a3521f20$ba9843d5 <@t> HISSWE.lokal>
Content-Type: text/plain;	charset="iso-8859-1"

Dear Paul,

In Europé it is two different mounting mediums(containing xylene),
Mountex and Pertex in the market. Both give you possibility to mount
direct after an extra jar with clean alcohol, these two mountingmedium
are very fast drying and do not change in colour after storing of many
years.
Eva 

-----Ursprungligt meddelande-----
Från: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] För Paul Bradbury
Skickat: den 5 oktober 2004 18:17
Till: HistoNet Server
Ämne: Re: [Histonet] Mounting media to covberslip from ethano


I have never encountered a mounting medium that uses alcohol as the 
solvent.

If one exists, two disadvantages spring to mind immediately. One, the 
refractive index of the medium would be low, so the clarity and 
brilliance associated with conventional, xylene-based media would be 
absent. Two, a large proportion of counterstains (eosin, van Gieson, 
neutral red, methylene blue, etc) are alcohol -soluble and would "bleed"

into the medium.

Paul


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------------------------------

Message: 9
Date: Tue, 05 Oct 2004 14:42:45 -0400
From: "Nicole Hedgecock" <n_hedgecock <@t> hotmail.com>
Subject: [Histonet] decalcified sections of bone
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY8-F78AnekVgKMy5K0001cd30 <@t> hotmail.com>
Content-Type: text/plain; charset=iso-8859-1; format=flowed

Hi, All

I am attempting to do TUNEL staining of osteocytes in cortical bone.  I was 
wondering why the bone has to be decalcified in order to do this process.  
And Why do the sections have to be so thin?

Thanks
Nicole



Nicole Hedgecock
Master's Student
Orthopedic Research Lab
UCD Medical Center
University of California, Davis

_________________________________________________________________
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Autos! http://latino.msn.com/autos/




------------------------------

Message: 10
Date: Tue, 05 Oct 2004 14:11:44 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Attn: Ann Featherstone, failed message delivery
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20041005141005.01b0a4b8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

To Ann Featherstone:

Message with mast cell staining protocols could not be delivered to your 
address, recontact me to get them again.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 11
Date: Tue, 05 Oct 2004 13:59:13 -0700
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: [Histonet] tissue processing
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <s162a8bc.079 <@t> ohsu.edu>
Content-Type: text/plain; charset=us-ascii

Histoneters,
I do microwave tissue processing, when tissue comes out, after the
paraffin and it is still mushy and has the feel of a sponge.  Does it
mean that I should keep in isopropyl alittle longer to make it alittle
firmer and that it isn't dehydrated all the way?  And paraffin is having
I hard time infiltrating the tissue?
 
Robyn
OHSU
Portland, Or


------------------------------

Message: 12
Date: Tue, 05 Oct 2004 21:01:10 +0000
From: "Thom Jensen" <tissuearray <@t> hotmail.com>
Subject: RE: [Histonet] Tissuearrays
To: Virginia.Achstetter <@t> afip.osd.mil,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY12-F237oDaSeXnbn000153b4 <@t> hotmail.com>
Content-Type: text/plain


   Hi Virgina,

   Have you looked on my website.  I have several articles in the journal
   of  histotechnology and some instructional videos on the same subjects
   that go into detail on the subject of array construction.

   My website is www.arrayworkshop.com

   1.  What  I do is place the array block on a slide face down.  Be sure
   to  put  the slide on it before you flip the block over.  Just in case
   some  of  the  cores  fall out of the block depending on how loose the
   cores are.

   2. Put the block ans slide (slide down) in an oven at approx. 37-40 'C
   for 15 to 20 minutes.

   3.  Press  the  slide  and  block  together.  You will notice that the
   paraffin  will  melt  a  little  and  the  paraffin  will  spread  out
   slightly.   This is good, because this is how the punches set into the
   block.

   4.  DO  NOT  seperate  the  slide  and block at this time.  Place then
   together  on  an  ice tray and allow to cool.  The slide will seperate
   easily once they are cold.

   5.  Before  cutting, trim the sides a little to make them straight and
   flat.  This will help the ribboning to go smoother with flat edges.

   6.   Do  not soak the block in ice water.  I have found that ice water
   makes  the punches swell thus making the tissue mushey.  Use only Ice.
   If  you  feel  you  might  get a better cut with water only soak for a
   minute or so.

   7.   Use  a fresh blade to make a ribbon.  I even knock down the blade
   with  a Kim wipe to help the cutting go quickly.  Running the kim wipe
   across  a  new blade removes oils also.  You will find  the knife make
   ribbons right away.

   The rest is normal histology techniques.  Lay the ribbons on the water
   bath, etc...


   If you have any more questions please feel free to email me.
   Thom Jensen

   HT (ASCP) / Array Technician




   >From: "Achstetter, Virginia A." <Virginia.Achstetter <@t> afip.osd.mil>
   >To: <histonet <@t> lists.utsouthwestern.edu>
   >Subject: [Histonet] (no subject)
   >Date: Tue, 5 Oct 2004 13:45:11 -0400
   >
   >Does  anyone  have  a good protocol for cutting Microarray blocks?  I
   understand that there are different methods of sealing the block cores
   so they stay put when sectioning.
   >
   >Ginny Achstetter HT (ASCP)
   >Armed Forces Institute of Pathology
   >Soft Tissue Pathology
   >6825 16th St. NW
   >Bldg 54 Rm. 3062
   >Washington, DC 20306
   >Fax: 202-782-9182
   >Phone: 202-782-1914
   >
   >_______________________________________________
   >Histonet mailing list
   >Histonet <@t> lists.utsouthwestern.edu
   >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
     _________________________________________________________________

   [1]Rock,  jazz,  country,  soul & more. Find the music you love on MSN
   Music!

References

   1. http://g.msn.com/8HMAENUS/2740??PS=47575


------------------------------

Message: 13
Date: Tue, 05 Oct 2004 17:42:55 -0400
From: "Sylvia Poulos" <spoulos <@t> saa.ars.usda.gov>
Subject: [Histonet] NADPH-TR protocol
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s162dd41.012 <@t> saa.ars.usda.gov>
Content-Type: text/plain; charset=US-ASCII

Hey all, 
Does anyone have a protocol for NADPH-tetrazolium reductase staining on
frozen tissues that they'd be willing to send me?
As always, thanks for all of the help! Sylvia

Sylvia P. Poulos
USDA-ARS-Animal Physiology Research Unit
Athens, GA 30605
706-583-8279
706-542-0399 (fax)



------------------------------

Message: 14
Date: Tue, 05 Oct 2004 17:44:50 -0400
From: "Nicole Hedgecock" <n_hedgecock <@t> hotmail.com>
Subject: [Histonet] More details: decalcified sections of bone
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY8-F107CErAWhJMYT00005449 <@t> hotmail.com>
Content-Type: text/plain; charset=iso-8859-1; format=flowed

I sent this out earlier (see below) and more details of my tissue processing 
and sectioning were requested.
I want to identify apoptotic osteocytes in rabbit cortical bone as well as 
some morphological features especially microcracks.  I fix the bones in 4% 
paraformaldehyde for 24 hours, then place then in 70% EtOH until I can 
decalcify them in EDTA at room temp.  Between the EtOH and EDTA steps I 
rinse the bones in dH2O for about 3 days.  After decalcification, the bones 
are again rinsed in dH2O and then embedded in paraffin and sectioned to 6 
microns.  I use the TUNEL kit from Roche for In situ labeling of DNA breaks.
Do you think that I have to do decalcification for the TUNEL to work? Does 
sectioning of paraffin embedded tissue create a lot of microscopic damage to 
the section?

I hope this clears up my situation and goals.
Nicole

>From: "Nicole Hedgecock" <n_hedgecock <@t> hotmail.com>
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] decalcified sections of bone
>Date: Tue, 05 Oct 2004 14:42:45 -0400
>
>Hi, All
>
>I am attempting to do TUNEL staining of osteocytes in cortical bone.  I was 
>wondering why the bone has to be decalcified in order to do this process.  
>And Why do the sections have to be so thin?
>
>Thanks
>Nicole
>
>
>
>Nicole Hedgecock
>Master's Student
>Orthopedic Research Lab
>UCD Medical Center
>University of California, Davis
>
>_________________________________________________________________
>¿Cuánto vale tu auto? Tips para mantener tu carro. ¡De todo en MSN Latino 
>Autos! http://latino.msn.com/autos/
>
>
>_______________________________________________
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------------------------------

Message: 15
Date: Tue, 5 Oct 2004 16:53:39 -0500
From: lkbauer <@t> unmc.edu
Subject: [Histonet] orienting mouse embryos
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF7982A5C3.ED6CFAF2-ON86256F24.00784495-86256F24.007844BD <@t> unmc.edu>
Content-Type: text/plain; charset=US-ASCII





Could I tap your collective experience to help solve a problem? I am trying
to orient gestational day 9.5 mouse embryos in paraffin for cross
sectioning. After processing, these specimens are like pieces of fragil
white pepper. I can only see head from tail with a microscope.  By the time
I get everything in focus the parffin is turning white....like finding a
ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin

Linda(Lin)Bauer
Department of Genetics, Cell Biology, and Anatomy
University of Nebraska Medical Center
Omaha, NE 68198-5455
Email: lkbauer <@t> unmc.edu




------------------------------

Message: 16
Date: Tue, 5 Oct 2004 18:10:01 -0400 
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] orienting mouse embryos
To: "'lkbauer <@t> unmc.edu'" <lkbauer <@t> unmc.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4092 <@t> fh2k093.fhmis.net>
Content-Type: text/plain;	charset="iso-8859-1"

Put eosin-phloxine stain (80ml to 3500ml) in your first alcohol when
processing, we use this on our biopsies and it makes all the difference!.
Janet

-----Original Message-----
From: lkbauer <@t> unmc.edu [mailto:lkbauer <@t> unmc.edu]
Sent: Tuesday, October 05, 2004 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] orienting mouse embryos






Could I tap your collective experience to help solve a problem? I am trying
to orient gestational day 9.5 mouse embryos in paraffin for cross
sectioning. After processing, these specimens are like pieces of fragil
white pepper. I can only see head from tail with a microscope.  By the time
I get everything in focus the parffin is turning white....like finding a
ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin

Linda(Lin)Bauer
Department of Genetics, Cell Biology, and Anatomy
University of Nebraska Medical Center
Omaha, NE 68198-5455
Email: lkbauer <@t> unmc.edu


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------------------------------

Message: 17
Date: Wed, 06 Oct 2004 08:18:08 +1000
From: Laurie Reilly <laurie.reilly <@t> jcu.edu.au>
Subject: Re: [Histonet] orienting mouse embryos
To: lkbauer <@t> unmc.edu, histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.1.0.14.0.20041006081451.00a39b30 <@t> mail.jcu.edu.au>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Dear Lin,
Try putting a few mls of aqueous eosin in your fixative. A pink specimen is 
a lot easier to see.
The eosin can be washed out at a later stage if it is not required.

                   Good luck and regards,   Laurie.


At 04:53 PM 10/05/04 -0500, lkbauer <@t> unmc.edu wrote:




>Could I tap your collective experience to help solve a problem? I am trying
>to orient gestational day 9.5 mouse embryos in paraffin for cross
>sectioning. After processing, these specimens are like pieces of fragil
>white pepper. I can only see head from tail with a microscope.  By the time
>I get everything in focus the parffin is turning white....like finding a
>ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin
>
>Linda(Lin)Bauer
>Department of Genetics, Cell Biology, and Anatomy
>University of Nebraska Medical Center
>Omaha, NE 68198-5455
>Email: lkbauer <@t> unmc.edu
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Mr.Laurie Reilly                                              Ph 07 4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.




------------------------------

Message: 18
Date: Tue, 5 Oct 2004 17:15:33 -0500
From: ajennings <@t> unmc.edu
Subject: Re: [Histonet] orienting mouse embryos
To: lkbauer <@t> unmc.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFADA3D269.3EEA7608-ON86256F24.007A460E-86256F24.007A4626 <@t> unmc.edu>
Content-Type: text/plain; charset=US-ASCII





Lin

Run downstairs and I will show you the tricks of the trade.....thought I
had already shown you :)




------------------------------

Message: 19
Date: Tue, 5 Oct 2004 19:01:18 EDT
From: Gervaip <@t> aol.com
Subject: [Histonet] Reusing the filter for ThinPrep for HPV
To: histonet <@t> pathology.swmed.edu
Message-ID: <110.398ec1e3.2e9481be <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Is anyone in HistoLand doing this?   We were told we can reuse the filter to 
prepare another slide for HPV.  If you do, how do you dry the filter?   

Pearl in New Orleans


------------------------------

Message: 20
Date: Wed, 6 Oct 2004 12:51:05 +1300
From: "Darren James" <darrenj <@t> medica.co.nz>
Subject: [Histonet] Bone Saw and Dust Extraction
To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000e01c4ab36$2e3140e0$c864a8c0 <@t> medica.co.nz>
Content-Type: text/plain;	charset="iso-8859-1"

Hello,

I have a question, does anyone know if there is an electric bone saw
available complete with a dust extraction unit for a Histology lab?
If not, would a saw with a water spray eliminate aerosol and particulate
matter?

I am looking for a benchtop saw for a histo lab not a Stryker or similar
mortuary saw.

Thanks

Darren James
Technical Representative

Medica Pacifica Ltd
Office Address: 1A 153 Stoddard Road, Mt Roskill, Auckland
Postal Address: PO Box 24-421, Royal Oak, Auckland 1030
Ph: +64 9 629 0823
Fax: +64 9 629 0542
Mob: + 64 21 633 820




------------------------------

Message: 21
Date: Tue, 5 Oct 2004 23:01:49 -0400 (EDT)
From: "SMITH,REBEKAH FELICIA" <rockbeki <@t> ufl.edu>
Subject: Re: [Histonet] DAB-colors...
To: "Louro, Pedro" <pedro.louro <@t> spcorp.com>,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<119304449.1097031709998.JavaMail.osg <@t> osgjas02.cns.ufl.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii

The only two colors I know of for DAB are brown and black. The 
black is due to adding nickel to DAB, I think. I think pierce 
sells them.
On Tue Oct 05 10:48:28 EDT 2004, "Louro, Pedro" 
<pedro.louro <@t> spcorp.com> wrote:

> Hello all,
> 
> When at NSH -Toronto..I heard people talking about using DAB in 
> different
> colors.
> Can someone tell who sells this product.
> 
> Thanks Pedro
> 
> 
> *********************************************************************
> This message and any attachments are solely for the intended 
> recipient. If you are not the intended recipient, disclosure, 
> copying, use or distribution of the information included in this 
> message is prohibited -- Please immediately and permanently 
> delete.
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 



--
SMITH,REBEKAH FELICIA
"You are a child of the universe, no less than the trees and the 
stars
You have a right to be here and whether or not it is clear to you, 
no doubt the universe is unfolding as it should. Therefore be at 
peace with G-d, whatever you conceive Him to be. And whatever your 
labors and aspirations,in the noisy confusion of life, keep peace 
in your soul.-Max Ehrmann,"Desiderata"




------------------------------

Message: 22
Date: Wed,  6 Oct 2004 08:46:37 +0200 (CEST)
From: Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
Subject: [Histonet] elastic fibers
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20041006064637.2BF5831C921 <@t> perceval.uca.es>
Content-Type: text/plain; charset="iso-8859-1"

Dear List fellows

Many thanks for all of you who answered to my question on elastic fibers stain. As I can see the more popular stains are in order of preference 1)Verhoeff's Van Gieson  2) Weigher resorcin fuchsin.

Greetings

José Luis


Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon <@t> icman.csic.es



------------------------------

Message: 23
Date: Wed, 6 Oct 2004 06:16:45 -0500
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
Subject: [Histonet] processing question
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <C124B59C51D3D447878265D53492834E0EFDD3 <@t> thimail.THI2.COM>
Content-Type: text/plain;	charset="us-ascii"

Hi histonetters,
 Is it harmful to tissue that is in 70% ETOH to be put on the processor
and start at 10% NBF?
Thanks .
Betsy Molinari HT(ASCP)
Texas Heart Institute
Houston,TX 77030
832-355-6524


------------------------------

Message: 24
Date: Wed, 6 Oct 2004 08:18:46 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Reusing the filter for ThinPrep for HPV
To: <Gervaip <@t> aol.com>,	<histonet <@t> pathology.swmed.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPAELECGAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain;	charset="us-ascii"

Pearl,
we routinely put the patient's filter in the Preservecyte vial after the
Thin prep is made. If we need o go back to that vial, we empty whatever
material is in the filter, back into the vial. Very carefully, we wipe both
sides of the filter with a Kimwipe and let the filter air dry (about a
minute. We try not to put too much pressure on the filter because sometimes
it comes off. Depending on the cellularity of the specimen, I have been able
to get 3 additional slides before I had to get another filter. I think they
clog up after a while.

Now, if anyone from Cytyc is listening, my rep is Billy Redding.

Disclaimer: This method is not approved by Cytyc, the FDA, the FCC, the FBI
and the CIA, the GOP, nor the DNC.

I will be expecting a phone call from Billy any minute now.


Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Gervaip <@t> aol.com
Sent: Tuesday, October 05, 2004 6:01 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Reusing the filter for ThinPrep for HPV


Is anyone in HistoLand doing this?   We were told we can reuse the filter to
prepare another slide for HPV.  If you do, how do you dry the filter?

Pearl in New Orleans
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 25
Date: Wed, 6 Oct 2004 06:49:19 -0700
From: Paula Lucas <plucas <@t> biopath.org>
Subject: [Histonet] Cassette Label System
To: "Histonet (E-mail)" <histonet <@t> pathology.swmed.edu>
Message-ID: <01C4AB70.9B2AB250.plucas <@t> biopath.org>

Hello,

My company is possibly interested in purchasing a histology cassette 
printer.

Could you please provide information on the different manufactures that 
offer printers?  What are the pro/cons?  Is it really that worth it to 
purchase one? Does it really save that much time?  Are they easy to use?

Any information, suggestions, and comments are greatly appreciated.

Thank you,

Paula Lucas
Bio-Path Medical Group




------------------------------

Message: 26
Date: Wed, 06 Oct 2004 08:48:23 -0500
From: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
Subject: [Histonet] Eye Protection Question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s163b17d.036 <@t> tvmdl.tamu.edu>
Content-Type: text/plain; charset=US-ASCII

Good Morning!

What kind of eye protection are technicians required to  wear in the laboratory during grossing, sectioning, processor maintenance, etc.?

Thanks and have a great day.

Jordan
TVMDL - College Station, TX






------------------------------

Message: 27
Date: Wed, 6 Oct 2004 09:22:40 -0500
From: Jackie.O'Connor <@t> abbott.com
Subject: Re: [Histonet] Eye Protection Question
To: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF220E2525.34EFD706-ON86256F25.004EAA97 <@t> northamerica.intra.abbott.com>
	
Content-Type: text/plain; charset="us-ascii"

Protection from biohaz splash where appropriate, protection from chemical 
splash, where appropriate.
As a note - just to walk into our labs here, even just to say hi to 
another person, we must have our safety glasses and labcoats on.  The 
thinking is that someone else working may create a splash that sails 
across the room into your eye. 




"Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
10/06/2004 08:48 AM

 
        To:     <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Eye Protection Question


Good Morning!

What kind of eye protection are technicians required to  wear in the 
laboratory during grossing, sectioning, processor maintenance, etc.?

Thanks and have a great day.

Jordan
TVMDL - College Station, TX




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 28
Date: Wed, 6 Oct 2004 10:55:37 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Cassette Label System
To: "Paula Lucas" <plucas <@t> biopath.org>,	"Histonet \(E-mail\)"
	<histonet <@t> pathology.swmed.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPIELFCGAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain;	charset="iso-8859-1"

Paula,
we just purchased a cassette printer from Surgipath. It prints one block at
a time, but can be set to automatically increment. We tested one printer
from TBS, but the cassettes kept jamming. The Surgipath printer maybe
slower, but it has less moving parts to break down and it does a great job.

Disclaimer: As usual, the opinions of the author in no way reflect the
opinions of the company, it executives or relatives.

Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Paula
Lucas
Sent: Wednesday, October 06, 2004 8:49 AM
To: Histonet (E-mail)
Subject: [Histonet] Cassette Label System


Hello,

My company is possibly interested in purchasing a histology cassette
printer.

Could you please provide information on the different manufactures that
offer printers?  What are the pro/cons?  Is it really that worth it to
purchase one? Does it really save that much time?  Are they easy to use?

Any information, suggestions, and comments are greatly appreciated.

Thank you,

Paula Lucas
Bio-Path Medical Group


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 29
Date: Wed, 6 Oct 2004 10:59:39 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Eye Protection Question
To: "Jordan Brod" <jbrod <@t> tvmdl.tamu.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPMELFCGAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain;	charset="US-ASCII"

Jordon,
we have full-face safety shields as well as surgical masks with an attached
eye shield. We wear either when changing the staining machines and tissue
processors. When I gross, I wear my eyeglasses that are classified as safety
wear. We have never had an incident, but we flush our eyewash stations
weekly because you never know what will happen.


Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jordan
Brod
Sent: Wednesday, October 06, 2004 8:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eye Protection Question


Good Morning!

What kind of eye protection are technicians required to  wear in the
laboratory during grossing, sectioning, processor maintenance, etc.?

Thanks and have a great day.

Jordan
TVMDL - College Station, TX




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 30
Date: Wed, 6 Oct 2004 12:40:36 -0400
From: "Fawn Jones" <fjones <@t> namsa.com>
Subject: [Histonet] micronucleus staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<915E55B02E236E4D95258B181EEF6317075D2B <@t> namsams01.namsa.int>
Content-Type: text/plain;	charset="us-ascii"

Hi everyone,
Our histology labs is doing a wright-giemsa stain on mice micronucleus
slides and we are having a problem with our cells not picking up the
stain.  Are there any suggestions for us.
We let them dry overnight then we put them in Methanol for 5 minutes,
let them air dry for 20 minutes then they go into Wright's stain for 6
minutes, then Wright-giemsa stain for 6 minutes, and then go through 3
changes of sterile water for injection for 30 seconds each.  Then they
sit overnight before coverslipping.  If anyone could help that would be
great.
Thanks
Fawn Jones
North American Science Associates


------------------------------

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 11, Issue 7
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