[Histonet] micronucleus staining

[Fawn Jones]

Hi everyone,
Our histology labs is doing a wright-giemsa stain on mice micronucleus
slides and we are having a problem with our cells not picking up the
stain.  Are there any suggestions for us.
We let them dry overnight then we put them in Methanol for 5 minutes,
let them air dry for 20 minutes then they go into Wright's stain for 6
minutes, then Wright-giemsa stain for 6 minutes, and then go through 3
changes of sterile water for injection for 30 seconds each.  Then they
sit overnight before coverslipping.  If anyone could help that would be
great.
Thanks
Fawn Jones
North American Science Associates