[Histonet] orienting mouse embryos

[Laurie Reilly]

Dear Lin,
Try putting a few mls of aqueous eosin in your fixative. A pink specimen is 
a lot easier to see.
The eosin can be washed out at a later stage if it is not required.

                   Good luck and regards,   Laurie.


At 04:53 PM 10/05/04 -0500, lkbauer <@t> unmc.edu wrote:




>Could I tap your collective experience to help solve a problem? I am trying
>to orient gestational day 9.5 mouse embryos in paraffin for cross
>sectioning. After processing, these specimens are like pieces of fragil
>white pepper. I can only see head from tail with a microscope.  By the time
>I get everything in focus the parffin is turning white....like finding a
>ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin
>
>Linda(Lin)Bauer
>Department of Genetics, Cell Biology, and Anatomy
>University of Nebraska Medical Center
>Omaha, NE 68198-5455
>Email: lkbauer <@t> unmc.edu
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Mr.Laurie Reilly                                              Ph 07 4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.