[Histonet] orienting mouse embryos

[Bonner, Janet]

Put eosin-phloxine stain (80ml to 3500ml) in your first alcohol when
processing, we use this on our biopsies and it makes all the difference!.
Janet

-----Original Message-----
From: lkbauer <@t> unmc.edu [mailto:lkbauer <@t> unmc.edu]
Sent: Tuesday, October 05, 2004 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] orienting mouse embryos






Could I tap your collective experience to help solve a problem? I am trying
to orient gestational day 9.5 mouse embryos in paraffin for cross
sectioning. After processing, these specimens are like pieces of fragil
white pepper. I can only see head from tail with a microscope.  By the time
I get everything in focus the parffin is turning white....like finding a
ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin

Linda(Lin)Bauer
Department of Genetics, Cell Biology, and Anatomy
University of Nebraska Medical Center
Omaha, NE 68198-5455
Email: lkbauer <@t> unmc.edu


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information contained in this message may be privileged and/or
confidential and protected from disclosure.  If the reader of this message
is not the intended recipient or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
dissemination, distribution or copying of this communication is strictly
prohibited.  If you have received this communication in error, please notify
the sender immediately by replying to this message and deleting the material
from any computer.