[Histonet] orienting mouse embryos

Bonner, Janet Janet.Bonner <@t> FLHOSP.ORG
Tue Oct 5 17:10:01 CDT 2004

Put eosin-phloxine stain (80ml to 3500ml) in your first alcohol when
processing, we use this on our biopsies and it makes all the difference!.

-----Original Message-----
From: lkbauer <@t> unmc.edu [mailto:lkbauer <@t> unmc.edu]
Sent: Tuesday, October 05, 2004 5:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] orienting mouse embryos

Could I tap your collective experience to help solve a problem? I am trying
to orient gestational day 9.5 mouse embryos in paraffin for cross
sectioning. After processing, these specimens are like pieces of fragil
white pepper. I can only see head from tail with a microscope.  By the time
I get everything in focus the parffin is turning white....like finding a
ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin

Department of Genetics, Cell Biology, and Anatomy
University of Nebraska Medical Center
Omaha, NE 68198-5455
Email: lkbauer <@t> unmc.edu

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