[Histonet] Mounting media/ethanol

[Cindy DuBois]

We use Clearium from  Surgipath and coverslip out of Isopropyl alcohol.  We have been doing this for several years.  One drawback we have seen is if they dried to quickly (ie. put in oven for drying), we see lots of bubbles.  We just put the flats on top of the embedding center for about 15 minuts and they are dry enough for the docs.  Then when we get them back we let them sit at room temp overnight, then put in over (60 C) overnight.
Another drawback is when using it with any alcohol soluble stain (Mucicarmine, Gram, etc.) the stain will leach out over time.  On these slides, we merely coverslip out of xylene.
 
Hope this helps,
Cindy DuBois, HT ASCP
Delta Pathology
Stockton CA

		
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