[Histonet] RE: Permanent Red

[C.M. van der Loos]

Hi Patty,
Indeed Dako's Permanent Red is a very nice chromogen that has a really crisp localization. However, we also found that it dissolves a bit in alcohol. The alcohol we used was not recycled and completely clean. According to the data sheet Permanent Red is not really designed to be solved in organic-based mountants. We let the slide dry completely on a hot plate (after washing with distilled water) and use VectaMount (H-5000). This is a non-aqueous mountant that works perfectly well on paraffin slides.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  Patti Loykasek <ploykasek <@t> phenopath.com> 
Date  Fri, 01 Oct 2004 07:38:18 -0700 
To  histonet <histonet <@t> pathology.swmed.edu> 
Cc  Bret Cook <bret.cook <@t> dakocytomation.com> 
Subject  [Histonet] Permanent Red 
Just a quick question on alternative chromogens. Has anyone had a problem
with Dako¹s Permanent Red coming out in recycled alcohols? This is a nice
alk. Phos. Chromogen, and I really like it. Occasionally though, it comes
out in the alcohols in our counterstaining/dehydrating set up ­ not every
time. We do use recycled alcohols in this set-up. If we switch the recycled
out for all fresh, we don¹t have this problem. Just wondering.

Patti Loykasek
PhenoPath Laboratories
Seattle, WA